Turk Lab Standard Operating Procedure (SOP) for apoptosis using TUNEL (TdT-mediated deoxyuridine-triphosphate nick end-labeling)
Materials Literature 1. Cut paraffin sections @ 4 microns and place on positively-charged slides (Surgipath X-tra Slides® 00210) 2. Microwave slides briefly 1-2 min. on high power until paraffin is just melted or bake in 60 degree C oven for 1 hour. 3. Use (Roche Diagnostics kit) positive control tissues inlcude lymph node, spleen, and small intestine.
Material List (top)
1. Surgipath X-tra Slides® 00210
3. Roche Diagnostics Kit
Literature (top)
Porcine reproductive and respiratory syndrome virus replicates in testicular germ cells, alters spermatogenesis, and induces germ cell death by apoptosis.
False positive staining in the TUNEL assay to detect apoptosis in liver and intestine is caused by endogenous nucleases and inhibited by diethyl pyrocarbonate.
C5b-9 terminal complement complex assembly on apoptotic cells in human arterial wall with atherosclerosis.
Colocalization of DNA fragmentation and caspase-3 activation during atresia in pig antral follicles.
1.
Cut paraffin sections @ 4 microns and place on positively-charged
slides (Surgipath X-tra Slides® 00210)